Effects of"Xiaobai Mixture"on Melanogenesis in Human Melanoma Cell line A375

Objective To observe the effects ofXiaobai Mixtureon the growth of human melanoma cell line A375,the activity of tyrosinase and the amount of Melanin. Methods The human melanoma cell A375 was divided into experimental groups in terms of various concentrations(0.01,0.1,1,10g/L) ofXiaobai Mixtureand control group.Then the proliferation of melanocytes,tyrosinase activity and melanic concentration were observed in vitro.MTT method was used to detect the proliferation of melanocytes;the tyrosinase activity of melanocytes was observed by zymologic method;the amount of melanin was observed by colorimetry (490nm).Results The proliferation of melanocytes,tyrosinase activity and melanic concentration were significantly increased by the treatment withXiaobai Mixtureat the concentrations of 0.01～10g/L.But it has inhibitory action when the concentration was continuously increased.ConclusionXiaobai Mixturehas bidirectional adjusting action in the growth of human melanoma cell line A375,suggesting that the dosage ofXiaobai Mixtureshould be optimized in clinical application.