Establishment of androgen-independent human prostate cancer cell line model LNCaP-AI

Objective:To establish an androgen-independent human prostate cancer cell line model LNCaP-AI.Methods:LNCaP cells were cultured in absence of hormone for a long-term to establish LNCaP cell line LNCaP-AI,which can live without hormone.MTT,immunofluorescence and RT-PCR techniques were used to study the proliferation activity of LNCaP-AI cells and expression and secretion level of PSA by LNCaP-AI cells in absence of hormone.Results:After cultured for 3 months,LNCaP cells gradually became accustomed to the non-hormone condition,showing the characteristics of androgen-independent LNCaP-AI cell line.LNCaP-AI cells rapidly proliferated under the non-hormone condition and secreted PSA.However,PSA mRNA expression level in LNCaP-AI cells was 44% that of the LNCaP cells under hormone condition.Conclusion:Androgen-independent LNCaP-AI cell line may simulate the development of androgen-independence in prostate cancer cells and is an ideal model of androgen-independent prostate cancer cell line.