Double protection for culturing SD rat bone marrow dendritic cells

Objective To establish an effective cell culture method for further investigating the biological function of the rat bone marrow dendritic cells(BMDC).Methods Rat BMDCs were induced by culture of rat bone marrow cells in the presence of both rrGM-CSF and rrIL-4 in vitro.The finally harvested cells were identified for morphology,phenotype and function.Results The final harvest cells displayed a typical dentritic cells morphology under the light microscope after 12 days of culture.The expression of the cell surface antigens increased in a time-dependent manner.Compared the molecular antigen expressions at different time,the difference was significantly(P 0.05).The capacity of the cultured cells to stimulate reactive T cell proliferation reached peak on the twelfth day of the culture.Conclusion This culture method is economical,practical and effective for induction and expansion of rat BMDC in vitro.