Factors Affecting Bulblet In Vitro Formation of Garlic

Micropropagation of garlic through bulblet formation in vitro can improve the survival rate of plantlet.As an experimental material,immature aerial bulblet can grow rapidly into(plant)lets under tissue cultural conditions.The experiment data suggested that plant growth regulators,pH value,sugar,temperature,photoperiod and genotype all affect the bulb formation to some extent.It seems that MS medium without phytohormone,5% of sugar,pretreatment at low temperature,long photoperiod and appropriate temperature(25℃) were favorable to bulblet formation.