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Aspirin induces apoptosis on human myeloid leukemia cell line HL-60

Yun Luo

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Abstract

Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy,TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells,and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.

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Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy,TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells,and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.

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Available abstract

Objective:To explore the effect of aspirin(ASA) on the apoptosis of human myeloid leukemia cell in vitro and its mechanisms.Methods:Inhibition rate was tested by MTT method.Apoptosis induced by ASA in HL-60 cells was investigated by applying light microscopy,TdT-mediated dUTP Nick-end Labeling(TUNEL) and flow cytometry analysis techniques.RT-PCR method were used to examine mRNA expression.Results:2.5～10.0mmol/L ASA can induce apoptosis and inhibit proliferation in HL-60 cells,and increase the sensitivity of HL-60 cells to Ara-c.ASA down-regulated the level of c-myc mRNA.Conclusion:A certain dosage of ASA can suppress the proliferation and induce apoptosis of acute leukemia cells in a dose dependent manner.One of the mechanisms of ASA inducing apoptosis may be related to down-regulated expression of c-myc mRNA.

Key concepts: Apoptosis, Myeloid leukemia, Flow cytometry, TUNEL assay, Leukemia, Molecular biology, Cell culture, In vitro

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