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2006•Journal of Fishery Sciences of ChinaRequires access

Preparation of antibodies against MCP of OGNNV

Xiaohong Chen, He Jianguo

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Abstract

Recombinant expression vector pET32a-MCP which inserted with MCP gene of OGNNV was transformed into BL21 for expression of MCP by inducing of IPTG.Polyclonal antibody to fusion protein was prepared from rabbits immunized with recombinant protein purified through column chromatography.The titre of anti recombinant protein sera was tested by ELISA.Western blot analysis confirmed specificity of the anti-sera.Results showed that the antibodies with the titre of 1∶22?000 could effectively neutralize OGNNV,and the relative percentage survival(RPS) of the fish injected with antibodies reached 54.50%.The antibodies was successfully prepared with purified recombinant MCP.Conclusion:which confirmed the immunogenicity of MCP from OGNNV and provided the scientific basis for anti-OGNNV vaccine of recombinant MCP.Also,the polyclonal antibodies can be used in further studies.

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What this paper is about

Recombinant expression vector pET32a-MCP which inserted with MCP gene of OGNNV was transformed into BL21 for expression of MCP by inducing of IPTG.Polyclonal antibody to fusion protein was prepared from rabbits immunized with recombinant protein purified through column chromatography.The titre of anti recombinant protein sera was tested by ELISA.Western blot analysis confirmed specificity of the anti-sera.Results showed that the antibodies with the titre of 1∶22?000 could effectively neutralize OGNNV,and the relative percentage survival(RPS) of the fish injected with antibodies reached 54.50%.The antibodies was successfully prepared with purified recombinant MCP.Conclusion:which confirmed the immunogenicity of MCP from OGNNV and provided the scientific basis for anti-OGNNV vaccine of recombinant MCP.Also,the polyclonal antibodies can be used in further studies.

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Available abstract

Recombinant expression vector pET32a-MCP which inserted with MCP gene of OGNNV was transformed into BL21 for expression of MCP by inducing of IPTG.Polyclonal antibody to fusion protein was prepared from rabbits immunized with recombinant protein purified through column chromatography.The titre of anti recombinant protein sera was tested by ELISA.Western blot analysis confirmed specificity of the anti-sera.Results showed that the antibodies with the titre of 1∶22?000 could effectively neutralize OGNNV,and the relative percentage survival(RPS) of the fish injected with antibodies reached 54.50%.The antibodies was successfully prepared with purified recombinant MCP.Conclusion:which confirmed the immunogenicity of MCP from OGNNV and provided the scientific basis for anti-OGNNV vaccine of recombinant MCP.Also,the polyclonal antibodies can be used in further studies.

Key concepts: Polyclonal antibodies, Recombinant DNA, Antibody, Immunogenicity, Molecular biology, Western blot, Titer, Fusion protein

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