2001•Zhongguo bingli shengli zazhiRequires access
The relationship between nitric oxide and gastric mucosal injury induced by reserpine in rats
MA Gao-feng
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Abstract
AIM: To study the relationship between nitric oxide (NO) and gastric mucosal injury induced by reserpine in rats. METHODS: Sixteen healthy male Wistar rats were randomly divided into control group and experimental group (n=8). NO contents and malondialdehyde(MDA) contents in plasma, gastric mucosa of the rats were respectively determined with Cadmium-reduct plus Greiss and TBA; nitric oxide synthase in gastric walls of the rats were observed using NADPH-diaphorase histochemistry and quantitatively measured with image analyzer.RESULTS: The NO contents in both plasma and gastric mucosa of experimental group were significantly lower than that in control group (P0 01),but their MDA contents were both higher than that in control group(P0 05,P0 01);the densities and A values of nitric oxide synthase (NOS)-positive nerve-cells and nerve fibers in gastric walls of experimental group were all obviously lower than that in control group (P0 05,P0 01). CONCLUSION: The mechanism of the reserpine-induced gastric mucosal injury in rats might be related to NO insufficiency arisen from the inhibition of NOS activity in NANC nerves in gastric wall,which might weaken the protection to gastric mucosa.