Simultaneous quantitative analysis of higher alcohol mixture with overlapping spectra

Objective To simultaneously determine the three-component system of isobutanol,isoamyl alcohol and n-propanol with overlapping spectra.Methods Experimental conditions were optimized according to the characteristics of color reaction of alcohols with paradimethylaminobenzaldehyde,and partial least squares(PLS) was use to simultaneously determine isobutanol,isoamyl alcohol and n-propanol with serious spectrum overlapping.Results The recoveries of simulative mixed samples were 99.8%,99.6% and 99.9% with RPEs 0.88%,1.81% and 0.90%,respectively.Recoveries and RPEs were within the allowable range.Conclusion Partial least squares method for the determination of three-component system of isobutanol,isoamyl alcohol and n-propanol was stable and reliable.