Study on the genes for beta-lactamases in Acinetobacter baumannii

Objective To study on the genes for beta-lactamases expression and spreading in Acinetobacter baumannii.Methods The Kirby-Bauer method were performed to detect the susceptibility of 11 kinds of antimicrobial agents against 17 strains isolated from blood samples.The genotypes of β-lactamases were analyzed by PCR and verificated by DNA sequencing.Results There were 10 multi-drug-resistance strains in 17strains.The resistant rates of 17strains to imipenam,meropenem and piperacillin/tazobactam were high,The sensitivity to cefoperazone / Sulbactam was the highest.In 17 strains of Acinetobacter baumannii TEM genes positive were 13 strains,OXA-23 genes positive were 10 strains,ADC genes positive were 12 strains.The other genes for beta-lactamases were negtive,10 strains TEM、OXA-23 andADC genes were all positive.Conclusions There are not only multi-drug-resistance but also high percentages of beta-lactamases genes(TEM,OXA-23 ADC etc.) in A.baumannii isolated from blood sample.OXA-23 genes for beta-lactamases procuring and spreading will be concerned with abuse β-lactama antimicrobial agents