2004Journal of Bengbu Medical CollegeRequires access

Study on staphylococcal enterotoxin B production and its gene of Staphylococcus aureus L-forms

J Guan, Xia PeiYing, Tang Su-lan

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Abstract

Objective:To investigate staphylococcal enterotoxin B(SEB) production and its gene of Staphylococcus aureus(S.aureus) L-forms.Methods:The production of SEB was examined by method ELISA and its gene was observed by PCR after S.aureus was induced into stable L-forms.Results:There was no significant difference in SEB production between S.aureus and its L-forms(P0.05),and positive band of (478 bp) was observed by PCR in S.aureus L-forms.Conclusions:SEB production is not changed and its gene is not lost after S.aureus is changed into L-forms.

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Objective:To investigate staphylococcal enterotoxin B(SEB) production and its gene of Staphylococcus aureus(S.aureus) L-forms.Methods:The production of SEB was examined by method ELISA and its gene was observed by PCR after S.aureus was induced into stable L-forms.Results:There was no significant difference in SEB production between S.aureus and its L-forms(P0.05),and positive band of (478 bp) was observed by PCR in S.aureus L-forms.Conclusions:SEB production is not changed and its gene is not lost after S.aureus is changed into L-forms.

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Available abstract

Objective:To investigate staphylococcal enterotoxin B(SEB) production and its gene of Staphylococcus aureus(S.aureus) L-forms.Methods:The production of SEB was examined by method ELISA and its gene was observed by PCR after S.aureus was induced into stable L-forms.Results:There was no significant difference in SEB production between S.aureus and its L-forms(P0.05),and positive band of (478 bp) was observed by PCR in S.aureus L-forms.Conclusions:SEB production is not changed and its gene is not lost after S.aureus is changed into L-forms.

Key concepts: Staphylococcus aureus, Enterotoxin, Microbiology, Medicine, Gene, Micrococcaceae, Staphylococcal infections, Bacteria

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