Construction of a Diagnostic cDNA Microarray for Serotype 2 Dengue Virus Detection

A fragment covering almost the full-length cDNA of dengue virus serotype 2 was amplified by long-PCR. Restriction endonucleases Sau3A I were utilized to digest the amplified products, thus a cDNA fragment library of dengue virus serotype 2 was constructed. Probes amplified from the cDNA library were designed and immobilized on chips by Pixsys 5500 Arrayer. Restriction Display method was used to label the samples when they were hybridized with the microarray. After hybridization,the microarrays were scanned with ScanArray Lite scanner. Hybridization results showed that there were no signals on the negative and blank controls, while obvious hybridization signals appeared on the spots of the positive controls and specific probes. It demonstrated that the diagnostic cDNA microarray was very sensitive and specific for detection of the serotype 2 dengue virus.