ScholarLens Back to search
2012•Food ScienceRequires access

Isolation, Purification and Characterization of Peroxidase from Chinese Chives

Tang Yun-ming

Open publisher page 0 citations

Abstract

Electrophoresis-pure peroxidase(POD) from Chinese chives was obtained after sample homogenization,extraction,ammonium sulfate precipitation,CM-Sepharose chromatography and Superdex-200 gel filtration.The purified POD had an activity of 14031.41U/mg.The purification factor was 102.96 and the recovery rate was 10.85%.The molecular weight of this enzyme was 28.4 kD,the optimum temperature and pH were 40 ℃ and 4.6,respectively.The POD enzyme was stable under 20-40℃ and pH 4-8.The Kmof this enzyme was determined to be 18.15 mmol/L under optimum conditions.Its activity could be strongly activated by low concentrations of oxalic acid,Zn2+and Mg2+,but inhibited by methanol,ethanol,isopropanol,SDS,ascorbic acid,Mn2+and Fe3+.

About this research paper

What this paper is about

Electrophoresis-pure peroxidase(POD) from Chinese chives was obtained after sample homogenization,extraction,ammonium sulfate precipitation,CM-Sepharose chromatography and Superdex-200 gel filtration.The purified POD had an activity of 14031.41U/mg.The purification factor was 102.96 and the recovery rate was 10.85%.The molecular weight of this enzyme was 28.4 kD,the optimum temperature and pH were 40 ℃ and 4.6,respectively.The POD enzyme was stable under 20-40℃ and pH 4-8.The Kmof this enzyme was determined to be 18.15 mmol/L under optimum conditions.Its activity could be strongly activated by low concentrations of oxalic acid,Zn2+and Mg2+,but inhibited by methanol,ethanol,isopropanol,SDS,ascorbic acid,Mn2+and Fe3+.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Electrophoresis-pure peroxidase(POD) from Chinese chives was obtained after sample homogenization,extraction,ammonium sulfate precipitation,CM-Sepharose chromatography and Superdex-200 gel filtration.The purified POD had an activity of 14031.41U/mg.The purification factor was 102.96 and the recovery rate was 10.85%.The molecular weight of this enzyme was 28.4 kD,the optimum temperature and pH were 40 ℃ and 4.6,respectively.The POD enzyme was stable under 20-40℃ and pH 4-8.The Kmof this enzyme was determined to be 18.15 mmol/L under optimum conditions.Its activity could be strongly activated by low concentrations of oxalic acid,Zn2+and Mg2+,but inhibited by methanol,ethanol,isopropanol,SDS,ascorbic acid,Mn2+and Fe3+.

Key concepts: Chemistry, Chromatography, Ammonium sulfate precipitation, Size-exclusion chromatography, Peroxidase, Oxalic acid, Ascorbic acid, Point of delivery

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Isolation, Purification and Characterization of Peroxidase from Chinese Chives — Research Paper | ScholarLens