Comparison of lung injury degrees after one-lung ventilation for different time

Objective To study the changes of oxygenation index,lung injury score and wet/dry lung weight ratio (W/D) after one lung ventilation for different duration on the rabbit models.Method 24 white Japanese rabbits were randomly divided into 4 groups (n=6). The control group performs two-lung ventilation for 2 h. The groups of O1,O2 and O3 performed one-lung ventilation for 1,2,3 h respectively, which were followed with two-lung ventilation for 1 h. Lung separation was achieved with a double-lumen tube made by myself. The arterial blood gas samples were drawn from femoral artery,the right pulmonary vein and the left one to get the oxygenation indexes. Light microscopic evaluations were performed to get the lung injury scores and W/D were determined. Result Lung injury scores were significant differences in left lung compared to right lung in the groups of O1,O2 and O3, though no significant differences were noted in the group C. Comparison of the right lungs of the four groups to all other documented significant increase of lung injury scores. The magnitudes of lung injury scores were related to OLV duration. The changes in the left lung were similar and more severe to the right ones. A similar changes of oxygenation index and W/D was found in all the groups.Conclusion Long time OLV could induced acute lung injury, which was inhomogeneous. The degrees of lung injury were related to OLV duration and were more severe in the non-ventilated lung.