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2007•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access

Extraction, purification and identification thyroglobulin of mouse

Fang Chen

Open publisher page 6 citations

Abstract

[Objective] To purify and identify thyroglobulin of mouse. [Method] Thyroid gland tissues were homogenized and salted out, and then purified by repeated gel filtration on Sphadex G-200. This protein was identified by SDS-PAGE and western Immunoblotting decting. [Result] This protein was verified as the high purified Thyroglobulin. [Conclusion] Sephadex G-200, together with Immunoblotting is a good way to purify and identify thyroglobulin of mouse.

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What this paper is about

[Objective] To purify and identify thyroglobulin of mouse. [Method] Thyroid gland tissues were homogenized and salted out, and then purified by repeated gel filtration on Sphadex G-200. This protein was identified by SDS-PAGE and western Immunoblotting decting. [Result] This protein was verified as the high purified Thyroglobulin. [Conclusion] Sephadex G-200, together with Immunoblotting is a good way to purify and identify thyroglobulin of mouse.

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Available abstract

[Objective] To purify and identify thyroglobulin of mouse. [Method] Thyroid gland tissues were homogenized and salted out, and then purified by repeated gel filtration on Sphadex G-200. This protein was identified by SDS-PAGE and western Immunoblotting decting. [Result] This protein was verified as the high purified Thyroglobulin. [Conclusion] Sephadex G-200, together with Immunoblotting is a good way to purify and identify thyroglobulin of mouse.

Key concepts: Thyroglobulin, Sephadex, Size-exclusion chromatography, Chromatography, Molecular biology, Thyroid, Chemistry, Protein purification

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