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2000•Chinese Journal of Geriatric Cardiovascular and Cerebrovascular DiseaseRequires access

Influence of angiotensin II and aldosterone on type-III collagen mRNA expression in cultured cardiac fibroblasts

Yi Zong

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Abstract

Objective The renin angiotensin aldosterone system (RAAS) plays a critically important role in the ventricular remolding.Type Ⅲ collagen is one of the major components of cardiac interstitium and contributes to cardiac fibrosis.The aim of this study was to determine whether RAAS influence the type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts.Methods Cell culture and RT PCR techniques were employed.Results Type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts was enhanced by angiotensin Ⅱ(10 -8 mol/L)and aldosterone(10 -8 mol/L),which could be abolished by corresponding antagonist saralasin and spironolactone respectively. Conclusions Angiotensin Ⅱ and aldosterone can enhance the type Ⅲ collagen mRNA expression directly.Their receptor antagonists may be useful in preventing cardiac fibrosis and remolding.

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Objective The renin angiotensin aldosterone system (RAAS) plays a critically important role in the ventricular remolding.Type Ⅲ collagen is one of the major components of cardiac interstitium and contributes to cardiac fibrosis.The aim of this study was to determine whether RAAS influence the type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts.Methods Cell culture and RT PCR techniques were employed.Results Type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts was enhanced by angiotensin Ⅱ(10 -8 mol/L)and aldosterone(10 -8 mol/L),which could be abolished by corresponding antagonist saralasin and spironolactone respectively. Conclusions Angiotensin Ⅱ and aldosterone can enhance the type Ⅲ collagen mRNA expression directly.Their receptor antagonists may be useful in preventing cardiac fibrosis and remolding.

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Available abstract

Objective The renin angiotensin aldosterone system (RAAS) plays a critically important role in the ventricular remolding.Type Ⅲ collagen is one of the major components of cardiac interstitium and contributes to cardiac fibrosis.The aim of this study was to determine whether RAAS influence the type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts.Methods Cell culture and RT PCR techniques were employed.Results Type Ⅲ collagen mRNA expression in cultured cardiac fibroblasts was enhanced by angiotensin Ⅱ(10 -8 mol/L)and aldosterone(10 -8 mol/L),which could be abolished by corresponding antagonist saralasin and spironolactone respectively. Conclusions Angiotensin Ⅱ and aldosterone can enhance the type Ⅲ collagen mRNA expression directly.Their receptor antagonists may be useful in preventing cardiac fibrosis and remolding.

Key concepts: Aldosterone, Spironolactone, Angiotensin II, Renin–angiotensin system, Internal medicine, Cardiac fibrosis, Endocrinology, Saralasin

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Influence of angiotensin II and aldosterone on type-III collagen mRNA expression in cultured cardiac fibroblasts — Research Paper | ScholarLens