Biochemical Analysis of Esterase from Housefly (Musca domestica) Resistant to Deltamethrin

Objective:To investigate the role of esterase in pyrethroid resistance of housefly.Methods: To detect the activity, kinetic parameters and the polyacryl gel electrophoresis spectrum of the esterase from the susceptible, resistant and super resistant strains. Results: The activity and the V m of the esterase in the three strains were similar. The K m of the esterase from resistant and super resistant strains was also similar. But the K m of the esterase in the susceptible strain was much higher. Further polyacryl gel electrophoresis revealed that the esterase from different strains involves different isoenzyme composition. Conclusion: The changes of esterase isoenzymes is involved in the mechanisms for pyrethroid resistance in housefly.