Effect of locally transplanted bone marrow mesenchymal stem cells committed induction on ischemic steletal masle

Objective To explore the effects of local autolougous transplantation of bone marrow mesenchymal stem cells induced by VEGF in vitro into ischemic skeletal muscles. Methods New Zealand Rabbits were randomizedly divided into two groups.Autologous bone marrow mesenchymal stem cells induced by 10 μg/L VEGF in vitro were injected into ischemic skeletal muscles after unilateral femeral artery and its branches were ligated and excised(n=8) .As a control, eight rabbits received only phosphate buffer solution (PBS) injection.Twenty-eight after the beginning of experiment,capillary density and apoptosis were detected,respectively. Results Twenty-eight after transplantation,capillary density was greater than that in control(P0.001).Ratio of the number of capillary and the number of muscle fiber was higher too(P0.05).Rate of apoptosis was significantly reduced in expanded cells group (P0.001). Conclusion It is a potential therapeutic method of peripheral ischemic disease by local transplantation of bone marrow mesenchymal stem cells.