Establishment and evaluation of collagen-induced arthritis rats model

Objective:To establish collagen-induced arthritis(CIA) rat model.Methods:Forty rats were randomly divided into two groups.The phlogistic cell factor TNF-α was detected by enzyme linked immunosorbent assay(ELISA),and the degree of pathogenicity as well as the pathological characteristics were assayed by histopathological techniques and joint roentgenography.Results:Compared with normal group,a peak arthritis index of the model group was achieved at 36 days after immunlization.The level of TNF-α analyzed by ELISA was significantly higher than that of the normal group(P0.05).As demonstrated by histopathology and roentgenography,typical arthritis pathology with chronic proliferative synovitis and secondarily destroyed articular cartilage and bone was found.Conclusion:The pathological characteristic of animal model with CIA was quite similar to that of human rheumatoid arthritis.This study provide a valuable experimental material for further study on the mechanism of pathogenesis and the clinical treatment of human rheumatic arthritis.