2003•Shenyang Yaoke Daxue xuebaoRequires access

Determination of the content of amino acid by pre-column derivatization and RP-HPLC

Ji Wang

Abstract

Objective To develop a pre-column derivatization procedure for the determination of the content of amino acid in the sample by reversed-phase high performance liquid chromatography.Method DNFB was used as a derivatization reagent and separation of DNP-amino acid derivatives were performed on an ODS column. The signals were detected at 360 nm with the UV detector. Result Under the obove conditions all the 14 amino acids in the standard solutions were separated to baseline in 30 min.Conclusion The method is applied to analyzing amino acid composition of ampeptide and good results are obtained.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Derivatization, Chromatography, Reagent, Chemistry, High-performance liquid chromatography, Amino acid, Organic chemistry, Biochemistry