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2006•Hu'nan Shifan Daxue xuebao. Ziran kexue banRequires access

Advanced Studies on the Human Gene ZNF322

Xiushan Wu

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Abstract

The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.The gene is located on Chromosome 6p22.1,and encodes a protein consisting of 402 amino acid residues and containing 9 tandem C_2H_2-type zinc-finger motifs.Northern blot results shows that the gene is expressed in all examined adult tissues.Subcellular location study indicates that ZNF322-EGFP fusion protein is distributed in the nucleus and cytoplasm.Reporter gene assays show that ZNF322 is a potential transcriptional activator.

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What this paper is about

The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.The gene is located on Chromosome 6p22.1,and encodes a protein consisting of 402 amino acid residues and containing 9 tandem C_2H_2-type zinc-finger motifs.Northern blot results shows that the gene is expressed in all examined adult tissues.Subcellular location study indicates that ZNF322-EGFP fusion protein is distributed in the nucleus and cytoplasm.Reporter gene assays show that ZNF322 is a potential transcriptional activator.

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Available abstract

The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.The gene is located on Chromosome 6p22.1,and encodes a protein consisting of 402 amino acid residues and containing 9 tandem C_2H_2-type zinc-finger motifs.Northern blot results shows that the gene is expressed in all examined adult tissues.Subcellular location study indicates that ZNF322-EGFP fusion protein is distributed in the nucleus and cytoplasm.Reporter gene assays show that ZNF322 is a potential transcriptional activator.

Key concepts: Gene, Complementary DNA, Zinc finger, Molecular biology, cDNA library, Subcellular localization, Biology, Fusion protein

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