2006•Journal of Zhengzhou UniversityRequires access
Effects of alcohol on mRNA expression of PPARγ and osteocalcin in human bone marrow mesenhymal stem cells
Xuejian Wu
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Abstract
Aim: To observe the effect of alcohol on the expression of adipogenic transcription factor (PPAR)γ mRNA and osteocalcin mRNA in human bone marrow mesenchymal stem cells (hBMSCs). Methods:Bone marrow was aspirated from the healthy adult human, and then the cells were cultured in vitro. The hBMSCs were isolated and obtained after the first passage, and then were divided into two groups. The cells were treated with or without 0.09 mol/L alcohol. The expression of PPARγ mRNA and osteocalcin mRNA in the hBMSCs of two groups was detected by RT-PCR technique. Results: The expression of PPARγ mRNA in the cells treated with alcohol (1.73±0.34) was significantlly higher than that in the cells treated without alcohol (1.38±0.27) (P0.05). The expression of osteocalcin mRNA in the cells treated with alcohol (0.48±0.11) was significantly lower than that in the cells treated without alcohol (1.08±0.22) ( P 0.05 ). Conclusion: Alcohol could induce higher PPARγ expression and lower osteocalcin expression of hBMSCs. which may involve in the alcohol-induced osteonecrosis.