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2011•Acta Agriculturae Boreali-SinicaRequires access

Diversity and Evolutionary Relationship of NBS-type Resistance Gene Analogues in Wheat

Daqun Liu

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Abstract

Degenerate primers based on conserved motif(P-loop and GLPL)of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames(ORFs) were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL.The phylogenetic analyses for the deduced amino acids showed that RGAs from wheat were nonTIR type.These results showed that NBS type RGAs isolated from wheat might have the same origin and mechanism of evolution as that in other plants.

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Degenerate primers based on conserved motif(P-loop and GLPL)of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames(ORFs) were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL.The phylogenetic analyses for the deduced amino acids showed that RGAs from wheat were nonTIR type.These results showed that NBS type RGAs isolated from wheat might have the same origin and mechanism of evolution as that in other plants.

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Available abstract

Degenerate primers based on conserved motif(P-loop and GLPL)of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames(ORFs) were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL.The phylogenetic analyses for the deduced amino acids showed that RGAs from wheat were nonTIR type.These results showed that NBS type RGAs isolated from wheat might have the same origin and mechanism of evolution as that in other plants.

Key concepts: ORFS, Genetics, Gene, Biology, Phylogenetic tree, Amino acid, Open reading frame, genomic DNA

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