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Effect of Acetylpuerarin on Apoptosis of Neuronal Cell and on Expression of PI3-K/Akt after Cerebral Ischemia and Reperfusion in Rats

Hou Dong-yu

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Abstract

Objective:To investigate the protective effect of acetylpuerarin on cerebral ischemia-reperfusion injury in rats and its mechanism.Method:Wistar rat was used as a model of focal cerebral ischemia and reperfusion.The positive reacted cells of apoptosis were counted and immunohistochemical method was used to measure the number of PI3-K/ Akt positive cells in rat's cerebral cortex of sham-operated group,model group,citicoline group and acetylpuerarin groups(10,50,250 mg·kg-1).Result:Acetylpuerarin could significantly increase the expression of the PI3-K and Akt,and reduce the number of cells of apoptosis(P0.01).The higher expression and the fewer cells of apoptosis in the medium dose acetylpuerarin group(P0.05)and the high dose acetylpuerarin group was better than the citicoline group(P0.05).Conclusion:Acetylpuerarin has obviously protective function for ischemia-reperfusion in rat.

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What this paper is about

Objective:To investigate the protective effect of acetylpuerarin on cerebral ischemia-reperfusion injury in rats and its mechanism.Method:Wistar rat was used as a model of focal cerebral ischemia and reperfusion.The positive reacted cells of apoptosis were counted and immunohistochemical method was used to measure the number of PI3-K/ Akt positive cells in rat's cerebral cortex of sham-operated group,model group,citicoline group and acetylpuerarin groups(10,50,250 mg·kg-1).Result:Acetylpuerarin could significantly increase the expression of the PI3-K and Akt,and reduce the number of cells of apoptosis(P0.01).The higher expression and the fewer cells of apoptosis in the medium dose acetylpuerarin group(P0.05)and the high dose acetylpuerarin group was better than the citicoline group(P0.05).Conclusion:Acetylpuerarin has obviously protective function for ischemia-reperfusion in rat.

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Available abstract

Objective:To investigate the protective effect of acetylpuerarin on cerebral ischemia-reperfusion injury in rats and its mechanism.Method:Wistar rat was used as a model of focal cerebral ischemia and reperfusion.The positive reacted cells of apoptosis were counted and immunohistochemical method was used to measure the number of PI3-K/ Akt positive cells in rat's cerebral cortex of sham-operated group,model group,citicoline group and acetylpuerarin groups(10,50,250 mg·kg-1).Result:Acetylpuerarin could significantly increase the expression of the PI3-K and Akt,and reduce the number of cells of apoptosis(P0.01).The higher expression and the fewer cells of apoptosis in the medium dose acetylpuerarin group(P0.05)and the high dose acetylpuerarin group was better than the citicoline group(P0.05).Conclusion:Acetylpuerarin has obviously protective function for ischemia-reperfusion in rat.

Key concepts: Citicoline, Apoptosis, Ischemia, Immunohistochemistry, Protein kinase B, Cerebral cortex, Reperfusion injury, PI3K/AKT/mTOR pathway

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Effect of Acetylpuerarin on Apoptosis of Neuronal Cell and on Expression of PI3-K/Akt after Cerebral Ischemia and Reperfusion in Rats — Research Paper | ScholarLens