Inhibition of proliferation and sensitization to chemotherapy of the osteosarcoma cells by survivin antisense oligonucleotide

Objective: To explore the inhibitiory effect of antisense oligodeoxynucleotide(ASODN) of survivin gene on the cultured osteosarcoma cell line MG63 and its sensitization effect to chemotherapy.Methods: survivin phosphorothioate ASODN was synthesized and transfected into MG63 cells by lipofectamine 2000.MTT assay was used to detect cell inhibition ratio.Apoptosis was observed by flow cytometry.Survivin mRNA and protein expression were determined by RT-PCR and Western blot respectively.Results: The proliferation of the cells transfected by lipofectamine 2000 was inhibited by survivin ASODN in a dose and time dependent manner.A higher total apoptosis rate(81.12±3.2)% could be induced in MG 63 cells in group Lip-ASODN than in group Lip-SODN(27.09±2.1)%(P(0.05)) and Group L(26.87±1.6)%(P0.05) after 72 h.The survivin mRNA and protein expression significantly decreased after treatment of survivin ASODN.The cell inhibition rate of the combination of survivin ASODN and ADM was higher significantly than ADM or survivin ASODN alone(both (P)0.05). Conclusion: Survivin ASODN is able to inhibit the proliferation of MG 63 cells,induce apoptosis,and enhance the sensitivity of MG63 cell to chemotherapy via down regulation of survivin expression.