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STUDY ON THE FERMENTATION CONDITIONS OF BACILLUS SUBTILIS PRODUCING THE NEUTRAL PROTEASE

DU Lian-xiang

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Abstract

The fermentation conditions of Bacillus subtilis which produces neutral protease were studied in this paper. The optimum fermentation medium consisted of 3 % soybean cake meal,3 % cornmeal,2.5 % wheat bran,0.05 % KH2PO4,0.4 % Na2HPO4. And the optimal culture conditions was the inoculum time 8 h,inoculum volume 2 %,poured solution volume 50 mL/ 250 mL,shake speed 180 r/min,temperature 32 ℃,culture time 33 h. Under the optimized conditions,the maximum enzyme activity reached 4 830 U/mL.

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What this paper is about

The fermentation conditions of Bacillus subtilis which produces neutral protease were studied in this paper. The optimum fermentation medium consisted of 3 % soybean cake meal,3 % cornmeal,2.5 % wheat bran,0.05 % KH2PO4,0.4 % Na2HPO4. And the optimal culture conditions was the inoculum time 8 h,inoculum volume 2 %,poured solution volume 50 mL/ 250 mL,shake speed 180 r/min,temperature 32 ℃,culture time 33 h. Under the optimized conditions,the maximum enzyme activity reached 4 830 U/mL.

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Available abstract

The fermentation conditions of Bacillus subtilis which produces neutral protease were studied in this paper. The optimum fermentation medium consisted of 3 % soybean cake meal,3 % cornmeal,2.5 % wheat bran,0.05 % KH2PO4,0.4 % Na2HPO4. And the optimal culture conditions was the inoculum time 8 h,inoculum volume 2 %,poured solution volume 50 mL/ 250 mL,shake speed 180 r/min,temperature 32 ℃,culture time 33 h. Under the optimized conditions,the maximum enzyme activity reached 4 830 U/mL.

Key concepts: Bacillus subtilis, Bran, Fermentation, Neutral protease, Protease, Food science, Volume (thermodynamics), Chemistry

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