Expression and effect of human ribosomal protein L6 on multidrug resistance in K562/A02 cells

Objective:To investigate the relationship between ribosomal protein L6 (RPL6) gene and the multidrug resistance of leukemia cell.Method:RPL6 cDNA was obtained by RT-PCR, Both sense and antisense cDNA recombinants of RPL6-encoding gene were constructed with pcDNA3.1(+) eukaryotic expression vector. The sense RPL6 cDNA recombinant was transfected into K562 cells while the antisense one into K562/A02 cells by liposomal reagent. The chemosensitivity of cells to adriamycin (ADM), Vincristine(VCR), Mitoxantrone (MIT) and Etoposide (VP16) was evaluated by MTT assay.Result:Internal control RT-PCR showed higher expression of RPL6 in K562/A02 than in K562 ( P 0.001 ); Sense-transfected K562 cells were 3.25 , 1.95 , 2.00 and 3.48 times more resistant to ADM, VCR, MIT and VP16 than control cells did ( P 0.05 or 0.01 ), whereas resistant to these drugs of antisense-transfected K562/A02 cells decreased by 62%, 50%, 56% and 47% than that of control cells ( P 0.05 or 0.01 ).Conclusion:RPL6 gene plays an important role in the development of drug resistance in K562/A02 cells.