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2012•Chinese Journal of Health Laboratory TechnologyRequires access

Analysis of SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an

Xiangyang Song

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Abstract

Objective: To investigate the SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an.Methods: Polymerase chain reaction(PCR) was used to determine the genotype of staphylococcal cassette chromosome mec(SCCmec).Results: All MRSA isolates were mecA positive.Among the 147 isolates of MRSA,1 isolate belonged to SCCmecⅡ,1 isolate belonged to SCCmecⅣa,144 isolates belonged to SCCmecⅢ and 1 isolate was not genotyped by this method.Conclusion: SCCmecⅢ genotype was the major epidemic isolates in Xi′an.

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What this paper is about

Objective: To investigate the SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an.Methods: Polymerase chain reaction(PCR) was used to determine the genotype of staphylococcal cassette chromosome mec(SCCmec).Results: All MRSA isolates were mecA positive.Among the 147 isolates of MRSA,1 isolate belonged to SCCmecⅡ,1 isolate belonged to SCCmecⅣa,144 isolates belonged to SCCmecⅢ and 1 isolate was not genotyped by this method.Conclusion: SCCmecⅢ genotype was the major epidemic isolates in Xi′an.

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Available abstract

Objective: To investigate the SCCmec genotype in methicillin-resistant Staphylococcus aureus in Xi′an.Methods: Polymerase chain reaction(PCR) was used to determine the genotype of staphylococcal cassette chromosome mec(SCCmec).Results: All MRSA isolates were mecA positive.Among the 147 isolates of MRSA,1 isolate belonged to SCCmecⅡ,1 isolate belonged to SCCmecⅣa,144 isolates belonged to SCCmecⅢ and 1 isolate was not genotyped by this method.Conclusion: SCCmecⅢ genotype was the major epidemic isolates in Xi′an.

Key concepts: SCCmec, Genotype, Staphylococcus aureus, Microbiology, Biology, Polymerase chain reaction, Methicillin-resistant Staphylococcus aureus, Meticillin

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