Effects of Repeated Dosing of 6% Hydroxyethyl Starch or 7.5% Sodium Chloride on Inflammatory Response and Oxidative Damage in Experimental Intracerebral Hemorrhage Rats

Objective To observe the effects of repeated dosing of 6% hydroxyethyl starch(130/0.4)or 7.5% sodium chloride on brain edema after experimental intracerebral hemorrhage(ICH)in rats.Methods167 male SD rats were divided into four groups randomly:Sham operation group(S,n=20),ICH control group(M,n=38),7.5% sodium chloride group(N,n=55)and 6% hydroxyethyl starch group(H,n=54).The model of the ICH was established with stereotactically infusing 50 μl of the autologous femoral artery blood into the right caudate nucleus.group N and group H received 7.5% sodium chloride 5 ml/kg and 6% hydroxyethyl starch 30 ml/kg at 2 h,24 h,48 h and 72 h after operation respectively.The tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),superoxide dismutase(SOD)and malondialdehyde(MDA)in the tissue around the hemorrhage were measured at different time point.ResultsThe IL-6 in group N was significantly more than that in group M at 24 h and 72 h after infusion(P0.05),and the TNF-α in group H was less than that in group M at 24 h and 48 h after infusion(P0.05).The SOD in group M decreased to the bottom at 48 h and 72h after ICH.SOD in group N and group H at 24 h,48 h and 72 h after infusion was both significant more than that in group M(P0.05).MDA in group H at 72 h after infusion was less than that in group M(P0.05).ConclusionRepeated infusion of 6% hydroxyethyl starch(130/0.4)or 7.5% sodium chloride can decrease inflammatory response of brain tissue after ICH,which may protect brain from oxidative damage.