2013•Journal of Apoplexy and Nervous DiseasesRequires access
Migration and localization of umbilical cord mesenchymal stem cells labeled by DAPI in the traumatic brain injury rat models
Zhiyu Li
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Abstract
Objective To observe the umbilical cord mesenchymal stem cell migration and positioning in model mice via the tail vein of human umbilical cord mesenchymal stem cell applications DAPI labeled transplanted into a mouse model.Methods HUC-MSCs were cultured in vitro.Good umbilical cord mesenchymal stem cells labeled with DAPI cultivate were injected into tail vein of a rat traumatic brain injury model mice;7 days after transplantation,the rats were sacrificed and brains were removed,distribution of mesenchymal stem cells and damage neurons in the model were observed using an inverted fluorescence microscope.Results The experiment successfully established a rat model of traumatic brain injury.DAPI marked umbilical cord mesenchymal stem cells were visible under inverted fluorescence microscope in model rat brain cortex and could fuse with damaged neuron.Conclusion Transplanted human umbilical cord mesenchymal stem cells could survival and migrate to the site of injury in the host body.