2006•Journal of Hepatobiliary SurgeryRequires access
METHYLATION OF SLIT2 GENE IN HEPATOCELLULAR CARCINOMA
Lixin Zhu
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Abstract
Objective To investigate methylation of SLIT2 gene in hepatocellular carcinoma. Methods Methylation-specific PCR was adopted to investigate methylation of SLIT2 gene in 50 cases of HCC and corresponding non-tumor liver tissue. 4 HCC cell lines ,8 normal liver tissue in liver angioma and 4 liver donors. The relationship between SLIT2 methylation and clinical data was analyzed. Result 4 liver donors and 8 normal liver tissue in liver angioma have no methylation. SLIT2 methylation was found in 3 of 4 HCC cell lines. In 50 HCC and non-tumor liver tissue,the frequency of SLIT2 methylation was 78%, 52%,respectively. SLIT2 methylation was more frequent in HCC than in non-tumor liver tissue (P=0. 006). SLIT2 methylation with TNM I was more frequent than that with TNM Ⅰ /Ⅱ (P = 0. 035). Half a year survival rates of HCC patients with SLIT2 methylation were not significantly lower than those without (P = 0. 208). Conclusion SLIT2 methylation is a frequent event in HCC and probably participates in the progression of HCC.