Preparation and identification of monoclonal antibody against human SOCS1

Objective To obtain and identify the monoclonal antibody(McAb) against human SOCS1.Methods BALB/c mice were immunized with recombinant GST-SOCS1 protein.Sp2/0 cells were fused with spleen cells of immunized mice.After screened and cloned repeatedly,the strain of hybridoma cell secreting anti-SOCS1 McAb was obtained.The specificity of anti-SOCS1 McAb was checked and evaluated with ELISA and Western blot.Its titer,immunoglobulin subtype and affinity were also measured.Results A strain of hybridoma cell secreting anti-SOCS1 McAb was successfully obtained and identified belong to IgG1 subtype.Its titers in cultural supernatant and ascetic fluid were 1:1280 and 1: 102400,respectively,by ELISA.Its affinity reached 1.17×107 L/mol.Conclusion The anti-SOCS1 McAb,which was successfully prepared,provides the foundation for further study on the negative regulation of cytokine signal transduction and the immuno-regulation of infected diseases caused by microorganisms.