2002•PubMedRequires access

[Construction and characterization of the cDNA library from Schistosoma japonicum cercariae].

Xiaoguang Chen, Hua Li, Hong‐Juan Peng, Shu-man Shen, M. C. Fung

Abstract

OBJECTIVE: To construct a cDNA library from Schistosoma japonicum cercariae. METHODS: Total RNA was extracted from 500,000 S. japonicum cercariae and used to synthesize the double-stranded cDNA and cloned into the TriplEx2 vector using "SMART cDNA Construction Kit". RESULTS: The primary titer of the constructed cDNA library is 1.8 x 10(7) pfu/ml and the titer of amplified library is 2.5 x 10(10) pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S. japonicum TPI and JF-2 genes was successfully amplified from the library. CONCLUSION: A cDNA library of S. japonicum cercariae has been constructed successfully.

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Key concepts: Schistosoma japonicum, cDNA library, Complementary DNA, Titer, Biology, Recombinant DNA, Molecular biology, Virology