Pharmacokinetic reseach of anti-cancer drug-Gemcitabine in vivo model by microdialysis technique

AIM:To estabalish the methodology of microdialysis technique in pharmacokinetic(PK) reseach of anticancer drugs in vivo. METHODS:Gemcitabine(GEM) was injected into mice's tail vein.The microdialysate samples from blood were collected after dosing in gemcitabine-treated mices.The concentration of GEM was detected real-time continuously and pharmacokinetic parameters were evaluated.RESULTS:The recovery rate of GEM in vivo was(11.9±2.0)%.It showed that the pharmacokinetics of gemcitabine was two-compartment model in vivo and the elimination and distribution of GEM meets the first kinetics.There were no serious side effects in model mice.CONCLUSION:Microdialysis can be successfully employed in living body to detect the concentration of GEM continuously,which prompts it is possible to study the PK of anticancer drugs in malignant tissues in vivo.