Study on Genetic Diversity of 60 Tomato Self-bred Lines with Good Quality Based on AFLP Markers
Sun Bao-jua
Abstract
Sun Bao-jua
Abstract
AFLP markers were used to evaluate genetic diversity among 60 tomato self-bred lines with good quality. Total 905 bands were amplified using 17 AFLP primer combinations,and 251 bands of them were polymorphic bands. Average number of polymorphic loci detected by per primer combination was 15,and mean polymorphism rate was 27. 7%. Unique fingerprints of 60 tomato self-bred lines were obtained by a minimum of 2 AFLP primer combinations,E7M4 and E7M10. Genetic similarity coefficients of 60 self-bred lines ranged from 0. 259 to0. 952 with a mean of 0. 664. 60 tomato materials can be divided into seven groups at similarity coefficients from0. 54 to 0. 57 when UPGMA clustering was used. It was showed that clustering results had no pronounced relation with phenotypic traits,such as sources,average weight per fruit,fruit shape,and so on.
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AFLP markers were used to evaluate genetic diversity among 60 tomato self-bred lines with good quality. Total 905 bands were amplified using 17 AFLP primer combinations,and 251 bands of them were polymorphic bands. Average number of polymorphic loci detected by per primer combination was 15,and mean polymorphism rate was 27. 7%. Unique fingerprints of 60 tomato self-bred lines were obtained by a minimum of 2 AFLP primer combinations,E7M4 and E7M10. Genetic similarity coefficients of 60 self-bred lines ranged from 0. 259 to0. 952 with a mean of 0. 664. 60 tomato materials can be divided into seven groups at similarity coefficients from0. 54 to 0. 57 when UPGMA clustering was used. It was showed that clustering results had no pronounced relation with phenotypic traits,such as sources,average weight per fruit,fruit shape,and so on.
Key concepts: Amplified fragment length polymorphism, UPGMA, Biology, Genetic diversity, Genetic similarity, Primer (cosmetics), Polymorphism (computer science), Horticulture