The Effect of Rosiglitazone on Proliferation and Apoptosis in Lung Cancer Cell Cultured in Vitro

Objective To investigate the selective effect of Rosiglitazone on proliferation and apoptosis in lung cancer cell line(A_(549)) cultured in vitro.Methods Human unbilical vein endothelial cell line HUVEC and lung carcinoma cell line(A_(549)) were used in this study.Cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tertrazolium blue(MTT) colorimetric assay.Cell cycle and apoptotic percentage were detected by flow cytometry.Expression of intracellular PPARγ protein was analyzed by indirect immunofluorescent flow cytometric technique.Results Rosiglitazone significantly inhibited A_(549) cell growth in a dose-dependent manner and a time-dependent manner.After treatment with Rosiglitazone,the enhanced G_1 arrest was accompanied with the enhanced apoptotic peak and percentage,and apoptotic peak and percentage in lung carcinoma cell line was significantly higher than those in human unbilical vein endotholial cell line HUVEC.Analysis on expression of intracellular PPARγ protein showed that Rosiglitazone could up-regulate PPARγ protein.Conclusions In the present study it is suggested that Rosiglitazone can inhibit the proliferation of lung carcinoma cell in vitro,its anticancer effect seems to be due to induction of apoptosis which may be a result of up-regulating PPARγ.