Breeding of white rot fungi from the decayed wood and preliminary study on the lignocellulose degradation property

The research purposes is to isolate and screen white rot fungi from the nature decayed wood,and analyse the ability to degrada lignocellulose.Using the selective medium enriched culture、PDA medium crossed separation preliminarily screened strains.Using microscope,the change of growth curve and the chromogenic reaction of lignin oxidase compound rescreened strains.The lignocellulose degradation ability of white rot fungi was analysed by the filter paper disintegration test and straw solid-state fermentation test.The results show that the morphological characteristics of bred B2 and E5 strains is in line with the biological characteristics of white rot fungi.Both of them have lignin oxidase and their filter weight loss rate can respectively reach 31.04% and 25.59% after cultivated 6 days,lignin degradation efficiency up to 33.7% and 30.7% after solid-state fermentation 20 days.B2 and E5 strains have the strong ability to degrade lignocellulose.The degradation effect of B2 strain is better than that of E5 strain.