Influence of CSA on Apoptosis Induced by SfaMNPV In SL-1 Cells

To investigate the effects of mitochondrial membrane permeability transition pore MPT-closed agent CSA on SfaMNPV induced apoptosis in SL-1 cell line. SL-1 cells were treated with SfaMNPV alone or in combination with CSA in different concentrations. Cell apoptosis was assessed by the morphological observation , DNA electrophoresis and flow cytometry. The mitochondrial transmembrane potentials (ΔΨm) were detected by flow cytometry according to the intensity of rhodamine 123 uptake in cells. CSA prevented SL-1 cells treated by SfaMNPV from apoptosis. CSA significantly restrained mitochondrial membrane potential of apoptotic SL-1 cells from degression. CSA restraining ΔΨm from degression possibly involves with CSA binding to CyP-D and CSA prevented SL-1 cells from apoptosis.