Effect of PDGF-BB on expression of estrogen receptor α protein of osteoblast in mature female rats

Objective To investigate the relationship between PDGF-BB and estrogen receptor-α (ER-α) and to elucidate the mechanism of the role of PDGF-BB in bone metabolism and the prospect of PDGF-BB in treatment of osteoporosis (OP).Methods The osteoblasts of adult female rat were separated by enzyme-digesting method and cultivated and its ER-α were identified. Different concentrations of PDGF-BB (0 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml) were applied to the cultural media for 7 days. The positive and negative contrast group were set for ER cells. ER-α protein was examined by Western blot.Results (1) The cells cultured were identified as osteoblast and ER-α was found in the nucleous by immunohistochemistry. (2) In SDS-PAGE electrophoresis, a clear protein stripe at 66kD was shown. (3) The protein stripes in Western blot in the group of 0.1 ng/ml, 1 ng/ml, 10 ng/ml were clearer than that of the group of 0 ng/ml and was the most in the group of 10 ng/ml.Conclusions PDGF-BB can increase the expression of ER-α in osteoblast and the concentration of ER-α protein increased with that of PDGF-BB.