Effect of specific ribozyme and mute ribozyme cleave on HBV mRNA in vitro

Objectives:To study the cleavage activity of multi target ribozyme anti HBV mRNA, and the effect of conserved base which was mutated to the activity of cleavage. Methods:Three connected ribozyme and mutated ribozyme against hepatitis B virus core gene were designed with computer, then constructed transcripitional vector of multi target ribozyme and mutated ribozyme which containing 5′,3′ self splicing ribozymes. The cleavage activity of ribozyme and mutated ribozyme on target RNA were analysed. Results:The ribozyme can be released from the self splicing vector after transcripitated.The ribozyme can specifically cleave different cleavage sites on the target mRNA.But the muta ribozyme can not cleave the target HBV mRNA. Conclusions:The connected ribozymes with computer design can cleave RNA of hepatitis B virus core gene.It is necessary of the conserved base for the cleavage activity of ribozyme.