Effects of rhizobium,soybean variety,soil type on nitrogenase activity

Considering the ecological environment in the main production areas in Heilongjiang Province, strains R1, R2 and R3 were inoculated to the varieties V1 and V2 in black soil and meadow soil in the pot experiment. In the stage of full bloom of soybean, the nitrogenase activity of excised root nodule was mensurated by acetylene reduction. The effect of single factor on nitrogenase activity was larger than that of factors, the interaction of strains and varieties was larger than that of others. The results indicated that treatment R1V2S2 and R1V1S1 had the highest nitrogenase activities, which were significantly higher than those of the two corresponding CK, respectively.