Influence of S1P on RPE cellα-SMA expression and RPE cell-induced collangen gel contraction in human

Objective To observe the influence of Sphingosine - 1 - phosphate(S1P) on expression ofα- smooth muscle acin(α-SMA) in human retinal pigment epithelium(vitreoretinopathy RPE) cells and RPE cell - induced contration of typeⅠcollagen gel.Methods RPE cells on conventional culture,take 3 - 4 on behalf of the mass of cells grow well for the experiments to 10~6/ ml were inoculated into 24 -well plate by adding serum -free medium containing S1P to S1P final concentration of 1,1×10~(-1),1×10~(-2),1×10~(-3),1×10~(-4)μg/ml,adding without S1P in serum - free culture medium for the control group,using immunofluorescence analysis of S1P on the expression ofα- SMA.Results 1,1×10~(-1),1×10~(-2)μg/ml S1P group to expressα- SMA - positive cells compared with the control group a clear statistical significance(P = 0. 000,0.0000,000),and 1×10~(-3),1×10~(-4)μg/ml group to expressα- SMA positive cells compared with the control group no statistically significant(P =0.832,0.781).Conclusionα- SMA mainly expresses in the cytoplasm of RPE cells.With the increasing in the concentration of S1P to enhance RPE cellα- SMA expression.