Inhibition of Angiotensin Converting Enzyme 2 by RNA Interference in Rat H9C2 Cell

Objective To screen the siRNAs that could inhibit the expression of angiotensin converting enzyme 2(ACE2) in rat H9C2 cell.Methods Three ACE2 siRNAs and one negative control siRNA were designed and synthesized and labeled with FAM at the 5'end for the measurement of transfection rate.siRNAs were transfected into H9C2 cells with Lipofectamine 2000.Realtime Quantity RT-PCR and Western-blot were performed to detecte the expression of ACE2 affected by the siRNAs at the level of gene and protein,respectively.Results The transfect rates of the three siRNAs and the negative control siRNA were all more than 95%.The siRNAs located in the 434 and 567 gene positions had no significant inhibition effect for ACE2(P0.05).However,the siRNA located in the 1205 gene position significantly decreased the expression of ACE2 at gene(decreased by 73.5%,P0.05) and protein levels(decreased by 70.0%,P0.01).Conclusion The siRNA located in the 1205 gene position could effectively inhibit the expression of ACE2 in rat H9C2 cells.