2005•Basic medical sciences and clinicsRequires access
Inhibition of BCR-ABL gene expression and induction of apoptosis of K562 cell lines by siRNAs
Yanbin Song, Wenli Ma, Chun-Qiong Feng, Xiangming Mao, Rong Shi, Zhang Bao, Zheng Wen-ling
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Abstract
Objective To study the inhibitory effect of BCR-ABL gene expression of K562 cell lines by RNAi technology.Methods Designing siRNAs targeting the breakpoint of BCR-ABL mRNA and transfecting K562 cells with lipofectamine~(TM) 2000,the inhibitory effect was observed by dedecting MTT、RT-PCR and FACS.Results The BCR-ABL expression obviously decreased and K562 cells had distinct apoptosis rate.Conclusions RNAi technology can inhibit BCR-ABL expression and induce apoptosis.