Susceptibility test of dermatophyte to four antifungal drugs using a modified M38-A protocol

Objective To test the susceptibility of clinical isolates of dermatophyte to four antifungal drugs with CLSI M38-A,and to evatluate its feasibility testing.Methods Modified M38-A protocol was used to determine the minimal inhibitory concentration(MIC) values of Econazole,Itraconazole Terbinafine and Voriconazole to all 31 isolates of dermatophyte,including 14 Trichophyton rubrum isolates,14 Trichophyton mentagrophytes isolates,1 Microsporum canis isolate,1 Epidermophyton floccosum isolate,and 1 Microsporum ferrugineum isolate.Results There were no significant differences in the susceptibility of Trichophyton rubrum and Trichophyton mentagrophytes to the drugs tested.While the MICs of Microsporum canis,Epidermophyton floccosum and Microsporum ferrugineum to any of the four drugs were higher than those of Trichophyton rubrum and Trichophyton mentagrophytes.Conclusion These data suggested that the modified M38-A protocol was reliable for antifungal susceptibility test of dermatophytes.