Removal of Sensory Cell Body Enhances the Long-Term Facilitation at Isolated Synapses of Aplysia

Objective To study the effect of vemoval of sensony cell body on long-term facilitation(LTF) induced by 5-HT applicaion at isolated synapses of aplysia.Methods Culture model of isolated synapses was established by using sensory neuron and motor neuron(L7) of aplysia,when the isolated synaptic model was cultured up to 4 days,some sensory cell bodies were cut for experiment,and rest of the cell bodies without cutting for control.Then a EPSP of isolated synapses was determined,and 5-HT was applied to the cultures,the cultures were kept in same method immediately after 5-HT application.The LTF at isolated synapse was evoked separately 24h and 48h after 5-HT treatment.Result Compared with control group witout cutting of cell body,LTF at isolated synapses of cutting group increased significantly 24h after 5-HT application,but decreased significantly and rapidly 48h after 5-HT treatment.Conclusion Removing sensory cell body facilitates the LTF at isolated synapses significantly 24h after 5-HT application,and decreased rapidly 48h.