2010•Hebei yiyaoRequires access
The influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line
Yang Lin
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Abstract
Objective To investigate the influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line.Methods The quantitative PCR was used to detect the expression of bcr/abl and SHIP2 gene in K562 cells after treated with Gleevic,and MTT and Western Blot were used respectively to detect the proliferation and phosphor-Akt levels of K562 cells after treated with Gleevic.Results With the increase of concentration of Gleevic and the prolangation of action time,the expression of bcr/abl gene was decreased in K562 cells,however,the SHIP2 gene expression was increased.The cell proliferation decreased and the levels of phosphor-Akt was decreased.Conclusion Gleevic can down-regulate the expression of bcr/abl gene.The expression of SHIP2 may be regulated by bcr/abl gene,which may affec cell proliferation by way of PI3K/AKT.