The Effect of different utilization on soil property and microorganism in sub-alpine meadow

The effect of controlled grazing, free grazing, cropping and degraded bare spot of subalpine meadow in Qilian Mountain on soil property and microorganism was studied in this paper The total amount of DNA of microorganisms in the soil was extracted by grinding and the LN Freezing and Melting method The analysis result showed that with grazing intensity increasing, grass yield and soil organic matter decreased, the dominant plant species changed, pH value was slightly higher and the total content of N and P decreased simultaneously These changes were all strongly correlated with organic matter The reduce of total DNA of soil microorganisms only occurred at bare spots Key wards:natural pasture;methods of utilization;degradation;soil;microorganism;DNA