Identification of Resistance to Sclerotinia Sclerotiorum in Soybean Germplasm

Sclerotinia stem rot of soybean,commonly called white mould,is a fungal disease caused by Sclerotinia sclerotiorum(Lib.)de Bery in the world.The disease distributed all throughout China,especially in Heilongjiang and Inner Mongolia.Heilongjiang is the main soybean production region in China,and white mould occurred seriously in recent years for the increase planting area of soybean,rape and sunflower.Utilization of resistant cultivars is the most economic,efficient,and safe method to control this disease.Screening on the resistance germplasm is the basis for resistance breeding.In this study,the reaction of 200 soybean lines to Sclerotinia sclerotiorum isolates Jia30 and Jian29 were evaluated by leafstalk inoculation in-vitro under controlled conditions.The results showed that there was no immunity accession among all the soybean lines tested,but there was difference in resistance among them.Four lines were resistant to the both isolates,representing 2% of all the accessions tested;Six lines were resistant to isolates Jia30,representing 3% of all the accessions tested;Ten lines were resistant to isolates Jian29,representing 5% of all the accessions tested.These lines could be used in soybean resistance breeding,and provide references for parent choosing in soybean resistant breeding and ecological control by arrangement of cultivars with different resistance.