Changes of MDA and SOD in ventilator-induced lung injury in rats

Objective To observe changes of MDA and SOD levels,and to study the effect of oxidative stress reaction in pathogenesis of ventilator-induced lung injury(VILI) in rats.Methods Twenty-four male Sprague-Dawley rats were randomly divided into two groups(n=12 each): group A received room air without mechanical ventilation,group B received large tidal volume with mechanical ventilation.After 4 h of ventilation,rats were sacrificed and the lung lavage liquid and lung tissue were collected and stored with correct methods.The measured indexes include total protein,WBC and TNF-α in bronchoalveolar lavage fluid(BALF),Wet-to-dry weight ratio(W/D) of lung,the levels of MDA,SOD in the blood and homogenate of harvested lung were assayed respectively.Lung pathological change was observed by microscope.Results Total protein,TNF-α and WBC in BALF and the levels of MDA in the lung and blood of group B were markedly higher than those of Group A(P0.01).The levels of SOD in the lung and blood of group B were significantly lower than those of group A(P0.01).Conclusion Strong oxidative stress is found in lung tissues of rats with VILI,and body′s anti-oxidative stress abilities is deficient relatively at the same.