Stathmin-specific siRNA expression plasmid inhibits the expression of stathmin

Objective Construct microtubule depolymerizing protein stathmin specific siRNA expression plasmid vector and study the biological function of stathmin in nasopharyngeal carcinoma.Methods Synthesize stathmin specific DNA fragments and anneal them to form double-strand DNA fragment.Subclone the DNA fragment into plasmid expression vector pGenesil-1.3.After the vector was amplified,it was identified by enzyme digestion and sequencing.Then,the identified recombinant plasmid vector was transferred into nasopharyngeal carcinoma CNE2 cell line by effectene transfection reagent of QIAGEN Company.Expression of stathmin was analyzed by RT-PCR and western blot.Results Enzyme digestion and sequencing data showed that siRNA expression plasmid vectors had been inserted stathmin-specific DNA fragment with right direction.Expression analysis confirmed that stathmin specific siRNA expression plasmid vector could effectively inhibit stathmin expression in nasopharyngeal CNE2 cells.Conclusion Recombinant stathmin specific siRNA expression plasmid vector can silence stathmin.